BAT3过表达缓解由PrP106-126毒性多肽诱导的神经元凋亡

doi:10.11843/j.issn.0366-6964.2015.05.022

ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (5): 841-848.doi: 10.11843/j.issn.0366-6964.2015.05.022

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Overexpression of BAT3 Alleviates Prion Protein Fragment PrP106-126-Induced Neuronal Apoptosis

SONG Zhi-qi^1# ，ZHAO Wen-jie^2# ，YANG Li-feng ¹，WANG Yun-sheng¹，ZHU Ting¹，CHENG Guang-yu ¹，ZHOU Xiang-mei¹，ZHAO De-ming ^1*

（1.State Key Laboratories for Agrobiotechnology，Key Lab of Animal Epidemiology and Zoonosis，Ministry of Agriculture，National Animal Transmissible Spongiform Encephalopathy Laboratory，College of Veterinary Medicine，China Agricultural University，Beijing 100193，China；2.College of Animal Husbandary and Veterinary Science，Henan Agricultural University，Zhengzhou 450000，China）

Received:2014-09-04 Online:2015-05-23 Published:2015-05-23

Abstract

Abstract:

The objective of this study was to investigate the interactions between BAT3 and prion protein and the potential role of BAT3 in PrP106-126-induced apoptosis.Immunofluorescence，the CCK-8 assay kit and western blotting were used to test the cytotoxicity of PrP106-126-FITC.After the stimulation of PrP106-126-FITC or PrP106-126，the location of BAT3 was tested by immunofluorescence and western blotting in the neurons.BAT3 was overexpressed in Neuro2a or primary neuronal cells by transfection with pcDNA3.1-HA-BAT3 expression plasmids.The effect of BAT3 on PrP106-126-induced cytotoxicity and apoptosis were detected by the CCK-8 assay and TUNEL assay.The expression of cytochrome c and Bcl-2 were examined by western blotting.Results were as follows：PrP106-126-FITC has similar cytotoxicity with PrP106-126.BAT3 interacted with prion protein and enhanced PrP expression.After PrP106-126 peptide treated，BAT3 was transported from the nucleus to cytoplasm，increased cell viability and protected neurons from PrP106-126-induced apoptosis through stabilizing the level of Bcl-2 protein and inhibiting the release of cytochrome c to cytoplasm.Our present data showed a novel molecular mechanism of PrP106-126-induced apoptotic process regulation through the overexpression of BAT3，which may be important for the basic regulatory mechanism of neuron survival in prion diseases and associated neurodegenerative diseases in vivo.

CLC Number:

S852.659.7

SONG Zhi-qi，ZHAO Wen-jie，YANG Li-feng,WANG Yun-sheng,ZHU Ting,CHENG Guang-yu,ZHOU Xiang-mei,ZHAO De-ming . Overexpression of BAT3 Alleviates Prion Protein Fragment PrP106-126-Induced Neuronal Apoptosis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(5): 841-848.

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Overexpression of BAT3 Alleviates Prion Protein Fragment PrP106-126-Induced Neuronal Apoptosis

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